Inclusion Criteria:
Patients must meet all of the following inclusion criteria to be eligible for inclusion
in the program:
1. Voluntary agreement to provide written informed consent and a willingness and
ability to comply with protocol requirements.
2. Male or female ≥18 years old.
3. Histologically, cytologically, or clinically confirmed diagnosis of unresectable or
metastatic Stage IIIb though IV/M1a through M1d cutaneous melanoma, as per AJCC
staging system, 8th edition.
4. Prior treatment with an anti-PD-1-containing regimen as monotherapy or in
combination (i.e., LAG-3).
5. Has ≥ 1 measurable and injectable lesion of ≥ 1 cm in longest diameter (or shortest
diameter for lymph nodes).
6. Has adequate hematologic function including:
1. White blood cell count ≥ 2.0 × 109/L. 2. Absolute neutrophil count ≥ 1.5 × 109/L. 3. Platelet count ≥ 75 × 109/L. 4. Hemoglobin ≥ 8 g/dL (without packed red blood cell transfusion within 2 weeks
of dosing)
7. Has adequate hepatic function, including:
1. Total bilirubin ≤ 1.5 × upper limit of normal (ULN; < 2.0 × ULN for patients
with known Gilbert syndrome or liver metastases)
2. Aspartate aminotransferase (AST) and alanine aminotransferase (ALT) ≤ 3.0 × ULN
(≤ 5.0 × ULN, if liver metastases are present)
3. Alkaline phosphatase (ALP) ≤ 2.5 × ULN (or ≤ 5.0 × ULN, if liver or bone
metastases are present)
8. Has adequate renal function, defined as serum creatinine ≤ 1.5 × ULN or creatinine
clearance ≤ 30.0 mL/minute/1.73 m2 (measured using Chronic Kidney Disease
Epidemiology collaboration [CKD-EPI] formula)
9. Prothrombin time (PT) ≤ 1.5 × ULN (or international normalization ratio [INR] ≤ 1.3)
and partial thromboplastin time (PTT) or activated partial thromboplastin time
(aPTT) ≤ 1.5 × ULN. Note: Patients which are on chronic anticoagulant therapy may be
enrolled if the target international normalized ratio (INR) is ≤ 2.5. for patients
requiring deep injection of VO, the INR must be < 1.5 at the time of injection.
10. Eastern Cooperative Oncology Group (ECOG) performance status (PS) 0 to 1.
11. Life expectancy of ≥ 3 months.
12. Male and female patients of reproductive potential must agree to avoid becoming
pregnant or impregnating a partner and adhere to highly effective contraception
requirement during treatment and for at 90 days after last dose of VO, or 5 months
after last dose of nivolumab.
13. Women of childbearing potential must have a negative serum beta-human chorionic
gonadotropin (β-hCG) test with a minimum sensitivity of 25 IU/L or equivalent units
β-hCG within 7 days before the first dose of program treatment.
Exclusion Criteria:
Patients meeting any of the following exclusion criteria will not be included in the
program:
1. Eligibility for, or previous randomization in the Replimune clinical trial
NCT06264180 (IGNYTE3 trial).
2. Prior treatment with anti-CTLA-4-directed therapy.
3. More than 2 lines of systemic therapy for advanced melanoma.
4. Known acute or chronic hepatitis B (defined as hepatitis B surface antigen [HBsAg]
reactive) or known acute or chronic hepatitis C virus (defined as HCV ribonucleic
acid [RNA] [qualitative] is detected). Note: Patients who have been effectively
treated are eligible for enrolment. Patients must be negative for HBsAg and HCV RNA.
5. Known human immunodeficiency virus (HIV) infection. Note: Testing for HIV is not
required unless mandated by local health authority or clinically indicated.
6. Active significant herpetic infections or prior complications of herps simplex virus
(HSV)-1 infection (e.g., herpetic keratitis or encephalitis) or requires
intermittent or chronic use of systemic (oral or intravenous [IV]) antivirals with
known antiherpetic activity (e.g., acyclovir). Note: Patients with sporadic cold
sores may be enrolled as long as no active cold sores are present at the time of
first dose of program treatment.
7. Had systemic infection requiring IV antibiotics or other serious active infection
requiring antimicrobial, antiviral, or antifungal treatment within 14 days before
dosing.
8. Known active central nervous system (CNS) metastases and/or carcinomatous meningitis
at time of screening. Patients with known CNS metastases are eligible if they have
received SOC therapy for CNS disease (such as stereotactic radiosurgery or radical
surgical resection followed by radiotherapy) and have disease stability on 2
subsequent scans performed at least at a 4-week interval.
9. Evidence of spinal cord compression or at high risk of spinal cord compression.
10. Serum lactate dehydrogenase > 2 × ULN.
11. Major surgery ≤ 2 weeks before starting program treatment.
12. No concurrent active malignancy requiring treatment.
13. History of significant cardiac disease including myocarditis or congestive heart
failure (defined as New York Heart Association Functional Classification III or IV),
or unstable angina, serious uncontrolled cardiac arrhythmia, cerebral vascular
accident, or myocardial infarction within 6 months from first dose of VO.
14. History of life-threatening toxicity related to prior immune therapy except those
that are unlikely to recur with standard countermeasures (e.g., hormone replacement
after adrenal crisis).
15. History or evidence of psychiatric, substance abuse (including IV substance abuse)
or any other clinically significant disorder, condition, or disease (with the
exception of those described above) that, in the opinion of the treating physician
would pose a risk to patient safety or interfere with the program evaluation,
procedures, or completion.
16. History or current evidence of any condition, therapy, or laboratory abnormality
that might confound the results of the program, interfere with the patient's
participation for the full duration, or is not in the best interest of the patient
to participate, in the opinion of the treating physician.
17. Active, known, or suspected autoimmune disease requiring systemic treatment.
18. History of (noninfectious) pneumonitis that required steroids or has current
pneumonitis.
19. Prior oncolytic virus therapy or other therapy given by intratumoral administration.
20. Was administered a live vaccine ≤ 28 days before the first dose of program
treatment.
21. Systemic anticancer therapies within 5 half-lives or 4 weeks of the first dose,
whichever is shorter.
22. Is currently participating in or has participated in a study of an investigational
agent within 4 weeks before the first dose of program treatment.
23. Has received prior radiotherapy and has not recovered from radiotherapy.
24. Conditions requiring treatment with immunosuppressive doses (> 10 mg per day of
prednisone or equivalent) of systemic corticosteroids other than for corticosteroid
replacement therapy 14 days before enrollment. Note: Patients who require a brief
course (≤ 7 days) or corticosteroids (e.g., as prophylaxis for imaging studies due
to hypersensitivity to contrast agents) are not excluded. Physiologic replacement
doses of systemic corticosteroids are permitted, only if the dose does not exceed 10
mg/day prednisone equivalent.
25. History of allergy or sensitivity to program drug components (VO, nivolumab) or
prior monoclonal antibody treatment.
26. Treatment with botanical preparations (e.g., herbal supplements or traditional
Chinese medicines) intended for general health support or to treat the disease under
study within 2 weeks before treatment.
27. Patient is deprived of freedom by an administrative or court order, or in an
emergency setting, or hospitalized involuntarily.
