Clinical Trial Finder

Inclusion Criteria:

1. Written informed consent and HIPAA authorization obtained from the subject/legal representative prior to performing any protocol-related procedures, including screening evaluations. 2. Subjects must be willing and able to comply with scheduled visits, treatment schedule, laboratory testing, and other requirements of the study, including disease assessment by MRI and tumor in situ fluid (TISF) collection. 3. Histologically confirmed diagnosis of glioblastoma. 4. Resection surgery done at the study center (Henan Provincial People's Hospital), with an reservoir intraoperatively implanted connecting the surgical cavity and the subscalp for postoperative noninvasive TISF collection. 5. Previous first line treatment with at least radiotherapy before the study treatment. 6. An interval of > 28 days and full recovery (i.e., no ongoing safety issues) from surgical resection prior to grouping. 7. Karnofsky performance status (KPS) of 70 or higher. 8. Life expectancy > 12 weeks.

Exclusion Criteria:

1. More than two recurrences of GBM. 2. Presence of extracranial metastatic, significant leptomeningeal disease or tumors primarily localized to the brainstem or spinal cord. 3. Any serious or uncontrolled medical disorder that, in the opinion of the investigator, may increase the risk associated with study participation or study drug administration, impair the ability of the subject to receive protocol therapy, or interfere with the interpretation of study results. 4. Subjects with active, known or suspected autoimmune disease. Subjects with vitiligo, type I diabetes mellitus, residual hypothyroidism due to autoimmune condition only requiring hormone replacement, psoriasis not requiring chronic and systemic immunosuppressive treatment, or conditions not expected to recur in the absence of an external trigger are permitted to enroll. Subjects have any other condition requiring systemic treatment with corticosteroids or other immunosuppressive agents within 14 days. Inhaled or topical steroids and adrenal replacement doses >10mg daily prednisone equivalent are permitted in absence of active autoimmune disease. 5. Previous radiation therapy with anything other than standard radiation therapy (i.e., focally directed radiation) administered as first line therapy. 6. Previous treatment with carmustine wafer except when administered as first line treatment and at least 6 months prior to randomization. 7. Previous bevacizumab or other VEGF or anti-angiogenic treatment. 8. Previous treatment with a PD-1, PD-L1 or CTLA-4 targeted therapy. 9. Evidence of > Grade 1 CNS hemorrhage on the baseline MRI scan. 10. Inadequately controlled hypertension (defined as systolic blood pressure ≥160 mmHg and /or diastolic blood pressure ≥100 mmHg) within 7 days of first study treatment. 11. Prior history of hypertensive crisis, hypertensive encephalopathy, reversible posterior leukoencephalopathy syndrome (RPLS) 12. Prior history of gastrointestinal diverticulitis, perforation, or abscess. 13. Clinically significant (i.e., active) cardiovascular disease, for example cerebrovascular accidents ≤ 6 months prior to study enrollment, myocardial infarction ≤ 6 months prior to study enrollment, unstable angina, New York Heart Association (NYHA) Grade II or greater congestive heart failure (CHF), or serious cardiac arrhythmia uncontrolled by medication or potentially interfering with protocol treatment. 14. Significant vascular disease (e.g., aortic aneurysm requiring surgical repair or recent arterial thrombosis) within 6 months prior to start of study treatment. Any previous venous thromboembolism ≥ NCI CTCAE Grade 3 within 3 months prior to start of study treatment. 15. History of pulmonary hemorrhage/hemoptysis ≥ grade 2 (defined as ≥ 2.5 mL bright red blood per episode) within 1 month prior to randomization. 16. History or evidence of inherited bleeding diathesis or significant coagulopathy at risk of bleeding (i.e., in the absence of therapeutic anticoagulation) 17. Current or recent (within 10 days of study enrollment) use of anticoagulants that, in the opinion of the investigator, would place the subject at significant risk for bleeding. Prophylactic use of anticoagulants is allowed. 18. Surgical procedure (including open biopsy, surgical resection, wound revision, or any other major surgery involving entry into a body cavity) or significant traumatic injury within 28 days prior to first study treatment, or anticipation of need for major surgical procedure during the course of the study. 19. Minor surgical procedure (e.g., stereotactic biopsy within 7 days of first study treatment; placement of a vascular access device within 2 days of first study treatment) 20. History of intracranial abscess within 6 months prior to randomization; 21. History of active gastrointestinal bleeding within 6 months prior to randomization. 22. Serious, non-healing wound, active ulcer, or untreated bone fracture. 23. Subjects unable (due to existent medical condition, e.g., pacemaker or ICD device) or unwilling to have a head contrast enhanced MRI. 24. Positive test for hepatitis B virus surface antigen (HBV sAg) or detectable hepatitis C virus ribonucleic acid (HCV RNA) indicating acute or chronic infection. 25. Known history of testing positive for human immunodeficiency virus (HIV) or known acquired immunodeficiency syndrome (AIDS) 26. History of severe hypersensitivity reaction to any monoclonal antibody. 27. Patients that require decadron > 4 mg/ day or equivalent of steroids

Primary study objectives: -To evaluate the clinical efficacy as measured by the overall survival (OS) rate at 12 months (Cohort 2) and OS rate at 18 months (Cohort 1). Secondary study objectives: -To evaluate the safety/tolerability of the study treatment; To compare the OS, progression-free survival and overall response rate of the two study groups. Exploratory objectives: -To evaluate the correlative biomarkers based on TISF ctDNA.

Arms

Experimental: Cohort 1

Subjects with ctDNA-level-relapse glioblastoma before clinical relapse, determined according to the dynamics of TISF ctDNA.

Experimental: Cohort 2

Subjects with clinical-relapse glioblastoma, determined according to the response assessment in neuro-oncology (RANO) criteria for gliomas.

Interventions

Drug: - Tislelizumab plus Bevacizumab

200mg sintilimab plus 3mg/kg bevacizumab every 3 weeks