Inclusion Criteria:
- - Female and/or male patients age >= 18 years.
- - Eastern Cooperative Oncology Group (ECOG) performance status (PS) 0, 1, or 2.
- - Patients must have at least 1 lesion (or aggregate lesions) to obtain tumor tissue
for resection of >= 1 cm or >= 4 core biopsies acceptable.
Amenable to image (CT,
ultrasound [U/S], or magnetic resonance imaging [MRI]) guided biopsy for tissue
collection necessary for neoantigen identification. Either primary or metastatic
sites are options for tissue collection.
- - Measurable disease by Response Evaluation Criteria in Solid Tumors (RECIST) 1.1
criteria: Participants must have measurable disease, defined as at least one target
lesion that can be measured in at least one dimension (longest diameter to be
recorded) as >= 10 mm, unless lymph node in which case short axis must be >= 15 mm.
Baseline imaging (for example diagnostic CT chest/abdomen/pelvis, PET CT scan and
imaging of the affected extremity as appropriate), brain imaging (MRI or CT scan)
must be obtained within 45 days of prior to start of first planned vaccine dose
infusion. MRI can be substituted for CT in patients unable to have CT contrast.
- - Serum creatine < 1.5 mg/dL or estimated glomerular filtration rate (eGFR) > 60
mL/min.
- - Total bilirubin (tBili) < 1.5 x upper limit of normal (ULN) and an aspartate
aminotransferase (AST)/alanine aminotransferase (ALT) < 2.5 x ULN and < 5 x ULN for
subjects with documented liver metastasis.
Patients with suspected Gilbert syndrome
may be included if tBili > 3 but no other evidence of hepatic dysfunction.
- - =< grade 1 dyspnea and arterial oxygen saturation (SaO2) >= 92% on ambient air.
If
pulmonary function tests (PFTs) are performed based on the clinical judgement of the
treating physician, patients with forced expiratory volume in 1 second (FEVI) >= 70%
of predicted and carbon monoxide diffusing capability (DLCO) (corrected) of >= 60%
of predicted will be eligible.
- - Patients with active interstitial lung disease (ILD)/pneumonitis or a history of
ILD/pneumonitis requiring treatment with systemic steroids will be excluded.
- - Patients 60 years of age or older are required to have left ventricular ejection
fraction (LVEF) evaluation performed within 60 days prior to enrollment.
LVEF may be
established with echocardiogram or MUGA scan, and left ejection fraction must be >=
50%. Cardiac evaluation for other patients is at the discretion of the treating
physician.
- - Subjects with a history of myocarditis or congestive heart failure (as defined by
New York Heart Association functional classification III or IV), as well as unstable
angina, serious uncontrolled cardiac arrhythmia, uncontrolled infection, or
myocardial infarction 6 months prior to study entry will be excluded.
- - Absolute neutrophil count (ANC) > 1000 cells/mm^3.
- - Platelet count >= 50,000/uL.
- - Toxicity from prior therapy must be recovered to National Cancer Institute (NCI)
Common Terminology Criteria for Adverse Events (CTCAE) version (v.
)5 grade 2 or less.
- - Willing and able to comply with scheduled visits, treatment plan, laboratory tests,
and other procedures.
- - Capable of understanding and providing a written informed consent.
- - The effects of neoantigen vaccination on the developing human fetus are unknown.
For
this reason, patients who are having sex that can lead to pregnancy must agree to
use adequate contraception (hormonal, barrier method of birth control, or
abstinence) for the duration of study participation. Should a woman become pregnant
while participating in the study, she should inform her study doctor immediately and
will not receive any more study treatment.
- - MELANOMA SPECIFIC: Tissue confirmation of melanoma: Histologically confirmed
metastatic (recurrent or de novo stage IV) or unresectable locally advanced (stage
IIIC or IIID) cutaneous, acral, conjunctival or mucosal melanoma, as defined by the
American Joint Committee on Cancer (AJCC) v8.0.
Confirmation of diagnosis must be or
have been performed by internal pathology review of archival, initial or subsequent
biopsy or other pathologic material at Fred Hutchinson Cancer Center
(FHCC)/University of Washington Medical Center (UWMC)
- - MELANOMA SPECIFIC: Patients must have received stage specific standard of care
therapy per National Comprehensive Cancer Network (NCCN) guidelines and have
persistent/recurrent disease after at least one line of therapy prior to enrollment
on the study.
- - MELANOMA SPECIFIC: Known BRAF mutational status.
- - MELANOMA SPECIFIC: History of detectable disease during/after treatment with a PD-1
or PD-L1 inhibitor, as defined by the Society of Immunotherapy of Cancer's
definition of primary or secondary resistance (Kluger and others [et al.
], 2020):
- - Drug exposure >= 6 weeks and best response progressive disease (PD) or stable
disease (SD) < 6 months or.
- - Drug exposure >= 6 months and best response complete response (CR), partial
response (PR), or SD > 6 months.
- - MELANOMA SPECIFIC: A confirmatory scan performed at least 4 weeks after disease
persistence/progression is required but this requirement can be waived if the
judgement of the treating clinician is that the patient would be at risk of rapid or
symptomatic progression in that interval.
This confirmatory scan can occur during
production of the vaccine after enrollment.
- - BREAST CANCER SPECIFIC: Tissue confirmation of stage IV (recurrent or de novo
metastatic) hormone receptor (HR) positive, HER2 negative breast cancer:
- Hormone receptor (HR) positive breast cancer as defined by either one, or both
of the following criteria:
- Estrogen receptor (ER) positive disease defined as follows documented by a
local laboratory: 1-100% positive stained cells based on de novo tumor
biopsy.
- - Progesterone receptor (PR) positive disease defined as follows documented
by a local laboratory: 1-100% positive stained cells based on de novo
tumor biopsy.
- - Human epidermal growth factor receptor 2 (HER2) negative breast cancer (per
American Society of Clinical Oncology [ASCO]/College of American Pathologists
[CAP] guideline update, 2018) as documented by a local laboratory with
HER2-negativity defined as:
- Immunohistochemistry score 0/1+ or 2+ and / or.
- - Negative by in situ hybridization (fluorescence in situ hybridization
[FISH]/chromogenic in situ hybridization [CISH]/silver-enhanced in situ
hybridization [SISH]) per ASCO/CAP guideline update, 2018.
- - Confirmation of diagnosis must be or have been performed by internal pathology
review of archival, initial or subsequent biopsy or other pathologic material
at FHCC/UWMC.
- - BREAST CANCER SPECIFIC: Patients must have received at least one line of systemic
therapy in the metastatic setting prior to enrollment on the study and have
progressive/persistent disease after.
- - NON-SMALL CELL LUNG CANCER SPECIFIC: Tissue confirmation of stage III unresectable
or stage IV (recurrent or de novo metastatic) non-small cell lung cancer (NSCLC):
- Genetic testing must have been performed for targetable driver mutations,
including EGFR, ROS1, Alk, KRAS, BRAF.
- - Confirmation of diagnosis must be or have been performed by internal pathology
review of archival, initial or subsequent biopsy or other pathologic material
at FHCC/UWMC.
- - NON-SMALL CELL LUNG CANCER SPECIFIC: Patients must have received at least one line
of systemic therapy in the metastatic or stage II or III setting including a PD-1 or
PD-L1 inhibitor prior to enrollment on the study and have progressive or recurrent
disease after.
- - NON-SMALL CELL LUNG CANCER SPECIFIC: For patients who have received neoadjuvant,
adjuvant, and/or consolidation anti-PD-1 or anti-PD-L1 for stage II or III disease,
they must have experienced disease progression in less than or equal to 365 days
from initiation (cycle 1 day 1) of anti-PD-1 or anti-PD-L1 therapy for this to count
as the systemic therapy for advanced disease.
Patients experiencing progression more
than 365 days from initiation (cycle 1 day 1) of anti-PD-1 or anti-PD-L1 therapy
will not be considered as having received one line of systemic therapy. These
patients must have received an anti-PD-1 or anti-PD-L1 therapy for stage IV or
recurrent disease.
Exclusion Criteria:
- - Fertile male patients and female patients of childbearing potential who are
unwilling or unable to use 2 highly effective methods of contraception as outlined
in this protocol for the duration of the study and for at least 5 months after the
last dose of investigational product.
- - Any history of an immune-related grade 4 adverse event attributed to prior cancer
immunotherapy CIT (other than endocrinopathy managed with replacement therapy or
asymptomatic elevation of serum amylase or lipase)
- Any history of an immune-related grade 3 adverse event attributed to prior CIT that
required permanent discontinuation of PD-1 inhibitor therapy.
- - Immune-related adverse events related to prior CIT (other than endocrinopathy
managed with replacement therapy or stable vitiligo) that have not resolved to
baseline.
Patients treated with corticosteroids for immune-related adverse events
must demonstrate absence of related symptoms or signs for >= 4 weeks following
discontinuation of corticosteroids.
- - Uncontrolled tumor-related pain.
Patients requiring narcotic pain medication must be
on a stable regimen at study entry. Symptomatic lesions amenable to palliative
radiotherapy (e.g., bone metastases or metastases causing nerve impingement) should
be treated > 4 weeks prior to enrollment. Patients should be recovered from the
effects of radiation.
- - Uncontrolled pleural effusion, pericardial effusion, or ascites requiring repeated
drainage more than once every 28 days.
Indwelling drainage catheters (e.g., PleurX®)
are allowed.
- - Patients with known symptomatic brain metastases.
Patients with previously diagnosed
brain metastases are eligible if they have completed their treatment and have
recovered from the acute effects of radiation therapy or surgery prior to study
entry, have discontinued corticosteroid treatment for these metastases for at least
4 weeks and are neurologically stable for >= 1 months (confirmed by magnetic
resonance imaging [MRI])
- - Patients with rapidly progressing disease, symptomatic visceral disease, or patients
who are expected to have rapidly progressive disease over the course of several
months despite bridging therapy approved by the protocol.
- - Known primary immunodeficiencies, either cellular (e.g., DiGeorge syndrome,
T-negative severe combined immunodeficiency [SCID]) or combined T- and B-cell
immunodeficiencies (e.g., T- and B-negative SCID, Wiskott-Aldrich syndrome, ataxia
telangiectasia, common variable immunodeficiency)
- Prior allogeneic bone marrow transplantation or prior solid organ transplantation.
- - Known positive test for HIV infection.
- - Patients with active infection causing fever (temperature > 38.1 degrees Celsius
[C]) or subjects with unexplained fever (temperature > 38.1 degrees C) may not
receive the investigational product unless the fever is =< 38.1 for 5 days prior to
start.
- - Active uncontrolled infection: individuals with a history of hepatitis C who have
successfully completed antiviral therapy with an undetectable viral load, and those
with hepatitis B who have, per standard practice, hepatitis well-controlled on
medication (e.g., AST and ALT < 5 x ULN) can be included.
- - History of autoimmune disease that has not been controlled with treatment in the
last 12 months, including, but not limited to, systemic lupus erythematosus,
rheumatoid arthritis, inflammatory bowel disease, vascular thrombosis associated
with antiphospholipid syndrome, Wegener granulomatosis, Sjogren syndrome,
Guillain-Barre syndrome, multiple sclerosis, vasculitis, or glomerulonephritis with
the following exceptions: Patients with a history of autoimmune hypothyroidism on a
stable dose of thyroid replacement hormone may be eligible.
Patients with controlled
type 1 diabetes mellitus on a stable insulin regimen may be eligible. Patients with
eczema, psoriasis, lichen simplex chronicus, or vitiligo with dermatologic
manifestations only (e.g., no psoriatic arthritis) may be eligible.
- - Treatment with systemic immunosuppressive medications (including, but not limited
to, prednisone > 10 mg/day or equivalent, cyclophosphamide, azathioprine,
methotrexate, thalidomide, and TNF-alpha antagonists) within 2 weeks prior
screening.
The use of topical, eye drops, local injections, or inhaled
corticosteroids (e.g. fluticasone for chronic obstructive pulmonary disease) is
allowed. The use of oral mineralocorticoids (e.g. fludrocortisone for patients with
orthostatic hypotension) is allowed. Physiologic doses of corticosteroids for
adrenal insufficiency are allowed. Low dose corticosteroids for a short duration [5
mg once daily (QD) prednisone for 2 weeks] as symptomatic treatment and upon with
discussion with the investigator is allowed. Note: Patients with adrenal
insufficiency may take 10 mg of prednisone or equivalent daily.
- - Subjects should have an international normalized ratio (INR) or activated partial
thromboplastin time (aPTT) =< 1.5 X ULN unless the subject is receiving
anticoagulant therapy.
Subjects on anticoagulant therapy should have a prothrombin
time (PT) or partial thromboplastin time (PTT) within therapeutic range of intended
use and no history of severe hemorrhage. Antiplatelet agents (eg, aspirin,
clopidogrel, etc.) are not considered anticoagulants for the purposes of this study
(i.e., they are allowed)
- - Other medical, social, or psychiatric factor that interferes with medical
appropriateness and/or ability to comply with study, as determined by the principal
investigator (PI)
- Participants of childbearing potential must have a negative serum pregnancy test
within 14 days prior to enrollment.
Childbearing potential is defined as women who
have not been surgically sterilized and who are not post-menopausal (free of menses
for at least 1 year)
- - Female patients who are lactating or intend to breastfeed during the duration of the
study.
- - Patients who have received a live vaccine within 30 days prior to enrollment.
- - Patients with any underlying medical condition for which, in the investigator's
opinion, participation would not be in the best interest of the participant (e.g.-
compromises the health of the subject) or that could prevent, limit or confound
protocol assessments.
- - MELANOMA SPECIFIC: Uveal or choroidal melanoma.
This entity is excluded due to the
absence of abundant mutations.
- - BREAST SPECIFIC: Patients with symptomatic disease including patients with
symptomatic lung metastases, bone marrow replacement with associated cytopenia, or
significant liver metastases with associated liver dysfunction.
- NON-SMALL CELL LUNG CANCER SPECIFIC: Activating mutations in EGFR or genetic
alterations in ROS1 or Alk, as these mutations are associated with lower mutation
burden and non-response to immune therapies
