Inclusion Criteria:
Subjects must meet all the following criteria to be enrolled in this study:
1. Age ≥18 years at the time of consent.
2. Written informed consent in accordance with national, local, and institutional
guidelines obtained prior to any study procedures. (Screening assessments performed
prior to informed consent but within the 28-day screening window are acceptable for
inclusion purposes).
3. Subjects must have histologically confirmed diagnosis of one of the following tumors:
triple negative adenocarcinoma of the breast (human epidermal growth factor receptor 2
negative, estrogen receptor negative and progesterone receptor negative
[HER2-/ER-/PR-]), adenocarcinoma of the colon or rectum, hepatocellular carcinoma
(HCC), osteosarcoma, epithelial ovarian cancer, malignant melanoma, non-small cell
lung cancer (NSCLC), or gastric cancer. Documentation of the diagnosis with the
original pathology report, or a recent biopsy, is required.
4. Subjects must have relapsed disease or refractory disease. Subjects must have
received, completed, or become intolerant of prior standard of care therapies or are
not expected to derive any clinical benefit from standard of care therapies.
5. Subjects with Ovarian cancer must have received at least one prior standard of care
for their relapsed or refractory disease, which must include a platinum-based regimen.
6. Subjects agree to provide fresh tumor tissue that has not been previously irradiated.
If biopsy procedure is not safe to perform, then archival tumor tissue (20 slides or a
tissue block) can be submitted.
7. Subjects must have iRECIST evaluable disease using computed tomography (CT) or
magnetic resonance imaging (MRI) with IV contrast, with at least one measurable target
lesion.
8. Subjects must have an Eastern Cooperative Oncology Group (ECOG) performance status of
0-1.
9. Subjects are expected to have a life expectancy of at least 12 weeks from the time of
enrollment.
10. Adequate hematologic function at the time of screening, defined as: absolute
lymphocyte count (ALC) >500 cells/mm3, absolute neutrophil count (ANC) >750 cells/mm3,
hemoglobin >8 g/dL, and platelet count >50,000 cells/mm3. For subjects enrolling into
the LDC cohorts, the criteria are defined as: ALC>500 cells/mm3, ANC>1000 cells/mm3,
hemoglobin>8g/dL, and platelet count>100,000 cells/mm3.
a. Hemoglobin and platelet count thresholds must be achievable without transfusion of
red blood cells or platelets, or use of growth factors administered within two weeks.
11. Adequate organ function, defined as:
1. Renal function: serum creatinine <1.5x institutional upper limit of normal (ULN)
or calculated creatinine clearance >50 mL/min.
2. Adequate hepatic function: total bilirubin ≤1.5x institutional ULN; aspartate
aminotransferase (AST) and alanine aminotransferase (ALT) ≤2.5x institutional
ULN, unless liver metastases are present, in which case it must be ≤5x ULN;
International Normalized Ratio (INR) ≤1.5. For subjects with HCC, adequate
hepatic function is defined as: total bilirubin ≤3x institutional upper limit of
normal, AST/ALT ≤5x institutional ULN, INR ≤1.7, Child-Turcotte-Pugh score <8.
12. Women of childbearing potential (defined as all subjects physiologically capable of
becoming pregnant) must have negative serum ß-human chorionic gonadotropin (hCG) or
urine pregnancy test.
13. Women of childbearing potential must agree to use highly effective methods of
contraception throughout the study and for six months after the last dose of CRX100.
14. Males who have partners of childbearing potential must agree to use an effective
barrier contraceptive method throughout the study and for six months after the last
dose of CRX100.
15. Subjects must be willing and able to comply with all study procedures, requirements,
and follow-up examinations.
Exclusion Criteria:
Subjects who meet any of the following criteria will be excluded from participation in this
study:
1. Subjects with new or progressive brain metastasis. Subjects with treated brain
metastases are eligible if there is no evidence of progression for at least four weeks
after central nervous system-directed treatment, as ascertained by clinical
examination and brain imaging (MRI or CT) during the screening period.
2. Subjects who received any chemotherapy or immunotherapies (non-cell-based therapies)
or oncolytic virus therapy, radiotherapy, radiosurgery or investigational agents
within three weeks of enrollment.
3. Subjects who still are experiencing Grade 2 or higher AEs from prior therapies such as
surgery, radiation therapy and systemic anti-cancer therapies unless approved by
sponsor.
4. Subjects who received any type of cell-based therapies within the last 12 weeks from
the planned apheresis date.
5. Subjects experiencing any active infections (bacterial, viral, or fungal) for which
systemic antimicrobials are required. Subjects who need prophylactic anti-viral agents
that can inhibit the replication of VACV will be excluded from participating.
6. Subjects must not have history of active or symptomatic autoimmune disease or evidence
of symptomatic autoimmune pneumonitis, glomerulonephritis, vasculitis, other
symptomatic autoimmune disease, documented history of autoimmune disease or syndrome
requiring systemic treatment in the past two years (i.e., with use of disease
modifying agents, steroids or immunosuppressive agents) Exceptions are permitted for
vitiligo, type I diabetes mellitus, residual hypothyroidism due to autoimmune
condition requiring only hormone replacement, psoriasis not requiring systemic
treatment, conditions not expected to recur in the absence of an external trigger or
other conditions approved by the medical monitor.
7. Have a condition requiring systemic treatment with either corticosteroids (>10 mg
daily prednisone or equivalent) or other immunosuppressive medications within 14 days
prior to apheresis, and within 14 days prior to infusion. Inhaled, intramuscular
injection, or topical steroids and adrenal replacement doses (≤10 mg daily prednisone
equivalents) are permitted. Stable doses of steroids are permitted for subjects with
pre-treated brain metastases. Short-term (<48 hr) steroid pretreatment for contrast
allergy for imaging is permitted.
8. Known human immunodeficiency virus (HIV) infection, active chronic hepatitis B or C,
life-threatening illnesses unrelated to cancer, or any serious medical or psychiatric
illness that could, in the Investigator's opinion, interfere with participation in
this study.
9. Pregnant or nursing an infant (subject or household contacts).
10. Clinically significant immunodeficiency (e.g., due to underlying illness and/or
medication) in a subject or household contacts.
11. Have any underlying medical condition (including, but not limited to, ongoing or
active infection requiring treatment, symptomatic congestive heart failure, unstable
angina pectoris, or cardiac arrhythmia), psychiatric condition that, in the opinion of
the Investigator, would compromise study administration as per protocol or compromise
the assessment of AEs.
12. Have a history of another invasive malignancy, except for the following circumstance:
individuals with a history of invasive malignancy are eligible if they have been
disease free and off treatment for at least two years or are deemed by the
Investigator to be at low risk for recurrence of that malignancy; individuals with the
following cancers are eligible if diagnosed and treated: carcinoma in situ of the
breast, oral cavity, or cervix, localized prostate cancer, or basal cell or squamous
cell carcinoma of the skin. When enrolling a subject with another malignancy, the
Investigator must discuss the subject with the Medical Monitor.
